Best time to initiate with bitch once heat starts

Hello I've looked and couldn't find exactly what I was looking for , my girl is almost 2 years and coming up on her 3rd heat cycle , we've played around a little out of heat and she has loved it , although I haven't been able to put my cock in her yet , I know that there are several stages of a heat cycle, but was curious at what point would be best to try to penetrate her finally
 
 
Do read the guide for many safety reasons, but the best indicator is when you lightly touch the area around her vulva and she moves her tail to the side decisively. There are also other signs to look for in the guide.
 
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